6SJV

Structure of HPV18 E6 oncoprotein in complex with mutant E6AP LxxLL motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-06
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 31 2 1
Unit cell lengths	106.825, 106.825, 100.377
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.792 - 2.029
Rwork	0.203
R-free	0.24240
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.792	2.102
High resolution limit [Å]	2.029	2.029
Number of reflections	82043	42645
<I/σ(I)>	14.4
Completeness [%]	98.9
Redundancy	9.8
CC(1/2)	0.999	0.563

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	298	Sodium Cacodylate 100 mM pH 6.5, PEG 8000 5%, 2-methyl-2,4-pentanediol 40%