6SHW
N-terminal domain of Drosophila X Virus VP3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-09-29 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.87260 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 77.331, 77.331, 48.054 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.043 - 2.000 |
R-factor | 0.1697 |
Rwork | 0.167 |
R-free | 0.22060 |
Structure solution method | AB INITIO PHASING |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | Arcimboldo |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.050 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.070 | |
Number of reflections | 6029 | 526 |
<I/σ(I)> | 34.49 | |
Completeness [%] | 98.5 | 88.51 |
Redundancy | 19.4 | |
CC(1/2) | 1.000 | 0.874 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 12% PEG 4K, 200 mM ammonium sulphate and 100 mM sodium acetate pH 4.6 |