6SEI

Recognition and processing of branched DNA substrates by Slx1-Slx4 nuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	P 1 21 1
Unit cell lengths	60.075, 92.700, 93.251
Unit cell angles	90.00, 91.60, 90.00

Refinement procedure

Resolution	46.610 - 2.690
R-factor	0.2152
Rwork	0.208
R-free	0.26550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6seh
RMSD bond length	0.004
RMSD bond angle	0.637
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.610	2.850
High resolution limit [Å]	2.690	2.690
Number of reflections	28286	4554
<I/σ(I)>	7.23	2.44
Completeness [%]	98.9	98.8
Redundancy	3.49	3.59
CC(1/2)	0.985	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298.15	0.1 M MES (pH 6.0), 0.2 M calcium chloride dihydrate, and 20% (v/v) PEG 6000