6SE5
Y830A mutant from Mycoplasma genitalium P110 adhesin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 98 |
| Detector technology | PIXEL |
| Collection date | 2019-02-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 109.322, 153.117, 169.902 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.300 - 2.210 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6r3t |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.220 |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.930 | 2.240 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.058 | 1.550 |
| Number of reflections | 70593 | 3502 |
| <I/σ(I)> | 12.5 | 0.6 |
| Completeness [%] | 98.8 | 99 |
| Redundancy | 4.6 | |
| CC(1/2) | 0.990 | 0.490 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 23 % PEG 3350 0.2 M Tri-potassium citrate |
