6SDK
Crystal structure of bacterial ParB dimer bound to CDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.186, 104.501, 103.193 |
| Unit cell angles | 90.00, 102.57, 90.00 |
Refinement procedure
| Resolution | 46.380 - 1.810 |
| R-factor | 0.1852 |
| Rwork | 0.184 |
| R-free | 0.21280 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.182 |
| Data reduction software | XDS (BUILT=20180126) |
| Data scaling software | XDS (BUILT=20180126) |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 1.800 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 174420 | 88506 |
| <I/σ(I)> | 11.1 | 0.8 |
| Completeness [%] | 99.8 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 5-16 % PEG 8000, 0.15-0.25 M calcium acetate or calcium chloride, and 50 mM Tris pH 7.5 or 8 |
