6SC9

dAb3/HOIP-RBR-HOIPIN-8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-06
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9763
Spacegroup name	I 2 2 2
Unit cell lengths	66.127, 87.052, 241.538
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	81.900 - 2.470
R-factor	0.2121
Rwork	0.210
R-free	0.25240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	dAb3/HOIP-RBR apo structure
RMSD bond length	0.002
RMSD bond angle	0.494
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	REFMAC (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.900	2.556
High resolution limit [Å]	2.467	2.467
Rmerge	0.049	0.701
Rmeas	0.069	0.992
Number of reflections	25600	2511
<I/σ(I)>	5.57
Completeness [%]	99.8
Redundancy	2
CC(1/2)	0.998	0.280

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	Ammonium sulfate, sodium chloride, HEPES