6SBS

YtrA from Sulfolobus acidocaldarius, a GntR-family transcription factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.979
Spacegroup name	P 62
Unit cell lengths	133.090, 133.090, 39.330
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.420 - 2.801
R-factor	0.2525
Rwork	0.249
R-free	0.32080
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.420	2.870
High resolution limit [Å]	2.801	2.801
Rmeas	0.209
Number of reflections	10103	727
<I/σ(I)>	8.88
Completeness [%]	100.0	100
Redundancy	10.01
CC(1/2)	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	20 % (w/v) 229 PEG3350, 0.2 M sodium nitrate and 0.1 M Bis-Tris propane, pH 8.5