6SBO
Estrogen receptor mutant L536S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.978570 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.430, 57.710, 87.800 |
| Unit cell angles | 90.00, 103.13, 90.00 |
Refinement procedure
| Resolution | 49.830 - 1.480 |
| R-factor | 0.191 |
| Rwork | 0.190 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.830 | 1.628 |
| High resolution limit [Å] | 1.480 | 1.484 |
| Number of reflections | 57289 | 2865 |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 88.5 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 22% PEG4000, NaCacodylate 0.1M pH 6.5, MgCl2 200mM |
