6SAX

Chromophore binding domain of bacteriophytochrome linked diguanylyl cyclase from Idiomarina species A28L (Pr-state monomer).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-08
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.873130
Spacegroup name	P 61
Unit cell lengths	149.960, 149.960, 77.520
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.894 - 2.400
R-factor	0.1995
Rwork	0.198
R-free	0.23590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5llw
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	53.894	53.894	2.500
High resolution limit [Å]	2.400	20.000	2.400
Rmerge	0.202	0.047	1.618
Rmeas	0.213	0.052	1.702
Total number of observations	395067
Number of reflections	38887	63	4453
<I/σ(I)>	9.69	34.71	1.57
Completeness [%]	99.7	80.8	99.5
Redundancy	10.159	7.46	10.418
CC(1/2)	0.996	0.996	0.559

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.24 M sodium malonate pH 7.0, 20 % PEG 3,350