6SAN
SALSA / DMBT1 / GP340 SRCR domain 8 soaked in calcium and magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2017-10-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.235, 46.643, 93.628 |
| Unit cell angles | 90.00, 97.37, 90.00 |
Refinement procedure
| Resolution | 30.950 - 1.360 |
| R-factor | 0.1909 |
| Rwork | 0.189 |
| R-free | 0.22550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6sa5 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.873 |
| Data scaling software | autoPROC (1.0.5) |
| Phasing software | MOLREP (CCP4Interface 7.0.056) |
| Refinement software | PHENIX (dev_3126) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.640 | 1.390 |
| High resolution limit [Å] | 1.360 | 1.360 |
| Rmeas | 0.074 | 0.801 |
| Number of reflections | 49168 | 4786 |
| <I/σ(I)> | 14.9 | |
| Completeness [%] | 98.3 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 50 % of 0.2 M MgCl2 hexahydrate, 20 % (v/v) isopropanol, 0.1 M HEPES, pH: 7.5. After crystal-formation, crystals were soaked in 10 mM MgCl2 and 10 mM CaCl2. |
