6SA1
Post catalytic complex of Prim-PolC from Mycobacterium smegmatis with gapped DNA and 3'-dUTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 198.250, 198.250, 85.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 88.660 - 2.010 |
| R-factor | 0.1855 |
| Rwork | 0.184 |
| R-free | 0.20420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5op0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.016 |
| Data reduction software | DIALS (1.7.3) |
| Data scaling software | xia2 (0.5.438) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | PHENIX (1.12-2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 99.120 | 99.250 | 2.040 |
| High resolution limit [Å] | 2.010 | 5.460 | 2.010 |
| Rmerge | 0.047 | ||
| Rmeas | 0.051 | 0.022 | 1.423 |
| Rpim | 0.020 | 0.009 | 0.594 |
| Total number of observations | 36427 | 31207 | |
| Number of reflections | 112811 | 6049 | 5562 |
| <I/σ(I)> | 20 | 66.6 | 1.2 |
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 6.4 | 6 | 5.6 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 285 | 50mM Sodium cacodylate, 2M Ammonium sulphate, 10mM Manganese Chloride |
