6SA0
Ternary complex of Prim-PolC from Mycobacterium smegmatis with 2nt gapped DNA and UpNHpp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 136.059, 136.059, 133.479 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.071 - 2.209 |
| R-factor | 0.1761 |
| Rwork | 0.174 |
| R-free | 0.20650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5op0 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.309 |
| Data reduction software | DIALS (1.5.0) |
| Data scaling software | xia2 (0.5.270) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | PHENIX (1.12-2829) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 117.830 | 118.000 | 2.250 |
| High resolution limit [Å] | 2.208 | 5.990 | 2.210 |
| Rmerge | 0.102 | ||
| Rmeas | 0.111 | 0.056 | 1.538 |
| Rpim | 0.043 | 0.023 | 0.595 |
| Total number of observations | 22917 | 22801 | |
| Number of reflections | 71872 | 3815 | 3518 |
| <I/σ(I)> | 8.5 | 18.2 | 1.5 |
| Completeness [%] | 100.0 | 100 | 99 |
| Redundancy | 6.4 | 6 | 6.5 |
| CC(1/2) | 0.996 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 285 | 50mM Sodium cacodylate, 2M Ammonium Chloride, 10mM Manganese Chloride |
