6S9K
Structure of 14-3-3 gamma in complex with caspase-2 peptide containing 14-3-3 binding motif Ser139 and NLS
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | LIQUID ANODE |
Source details | Excillum MetalJet D2+ 70 kV |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-26 |
Detector | Bruker PHOTON II |
Wavelength(s) | 1.3418 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 59.790, 78.230, 122.500 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.720 - 1.600 |
R-factor | 0.2041 |
Rwork | 0.203 |
R-free | 0.22120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b05 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.570 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.720 | 1.657 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmeas | 0.193 | 0.768 |
Number of reflections | 38244 | 3743 |
<I/σ(I)> | 19.34 | |
Completeness [%] | 99.9 | |
Redundancy | 9.13 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | PEG 400, calcium chloride, HEPES, 1,1,1,3,3,3-hexafluoropropan-2-ol |