6S9K
Structure of 14-3-3 gamma in complex with caspase-2 peptide containing 14-3-3 binding motif Ser139 and NLS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | Excillum MetalJet D2+ 70 kV |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-26 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.3418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 59.790, 78.230, 122.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.720 - 1.600 |
| R-factor | 0.2041 |
| Rwork | 0.203 |
| R-free | 0.22120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b05 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.570 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.720 | 1.657 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmeas | 0.193 | 0.768 |
| Number of reflections | 38244 | 3743 |
| <I/σ(I)> | 19.34 | |
| Completeness [%] | 99.9 | |
| Redundancy | 9.13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | PEG 400, calcium chloride, HEPES, 1,1,1,3,3,3-hexafluoropropan-2-ol |
