6S9A
Artificial GTPase-BSE dimer of human Dynamin1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.2825 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 70.693, 69.546, 74.277 |
| Unit cell angles | 90.00, 113.15, 90.00 |
Refinement procedure
| Resolution | 48.730 - 1.860 |
| R-factor | 0.2194 |
| Rwork | 0.219 |
| R-free | 0.25450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5d3q |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.442 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.730 | 1.970 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 55429 | 17351 |
| <I/σ(I)> | 9.02 | |
| Completeness [%] | 98.5 | 98.3 |
| Redundancy | 3.49 | 3.47 |
| CC(1/2) | 0.999 | 0.216 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M Tris-HCl, 0.25M KBr, 30% (w/v) PEG MME 2000 |
