6S99
Dimethylated fusion protein of RSL and trimeric coiled coil in complex with cucurbit[7]uril
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-21 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.219, 51.242, 111.282 |
| Unit cell angles | 76.80, 89.88, 60.05 |
Refinement procedure
| Resolution | 42.842 - 2.649 |
| R-factor | 0.2262 |
| Rwork | 0.225 |
| R-free | 0.25650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.364 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.842 | 2.695 |
| High resolution limit [Å] | 2.649 | 2.649 |
| Rmerge | 0.056 | 0.313 |
| Rpim | 0.035 | 0.190 |
| Number of reflections | 26787 | 1383 |
| <I/σ(I)> | 7.8 | 2.7 |
| Completeness [%] | 97.4 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M TRIS HCL pH 8.5, 20% PEG8000, 0.2 M Magnesium Chloride, 0.0013 M cucurbit[7]uril |
