6S8Z
Elongation Factor P from Corynebacterium glutamicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-05 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.968 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.201, 38.163, 55.356 |
| Unit cell angles | 90.00, 93.09, 90.00 |
Refinement procedure
| Resolution | 38.580 - 2.200 |
| Rwork | 0.206 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oyy |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.721 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.580 | 2.279 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.129 | 2.355 |
| Rmeas | 0.145 | 2.631 |
| Rpim | 0.065 | 1.158 |
| Number of reflections | 10932 | 1081 |
| <I/σ(I)> | 7.98 | 0.63 |
| Completeness [%] | 98.6 | 98.9 |
| Redundancy | 4.9 | 5.1 |
| CC(1/2) | 0.996 | 0.613 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M Sodium acetate 0.1 M HEPES pH 7.5 22 %(w/v) PEG 4000 |
