6S8X
Crystal structure of the Rab-binding domain of FIP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-12 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 62.538, 68.430, 172.086 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.020 - 2.290 |
| R-factor | 0.2473 |
| Rwork | 0.246 |
| R-free | 0.27390 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.778 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.160 | 2.370 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.111 | 0.872 |
| Rmeas | 0.126 | 1.048 |
| Rpim | 0.059 | 0.483 |
| Number of reflections | 16934 | 1611 |
| <I/σ(I)> | 8.8 | 1.7 |
| Completeness [%] | 99.4 | 99.3 |
| Redundancy | 4.3 | 4.3 |
| CC(1/2) | 0.712 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.01M cobat chloride 0.1M NaOAc, pH 4.7 1M 1,6 hexanediol |
