6S82
Structure Of D80A-Fructofuranosidase From Xanthophyllomyces Dendrorhous Complexed With Tris-buffer molecule And hydroquinone
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979490 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 74.579, 205.623, 145.369 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.940 - 1.850 |
| R-factor | 0.1552 |
| Rwork | 0.154 |
| R-free | 0.17530 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5ann |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.755 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.940 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.079 | 0.559 |
| Rpim | 0.032 | 0.227 |
| Number of reflections | 190741 | 64488 |
| <I/σ(I)> | 14.5 | 2.9 |
| Completeness [%] | 100.0 | |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.3 M Sodium Citrate tribasic dihydrate, 0.1M TRIS-HCl pH 8.5, then soaked in hidroquinone |
