6S7V

Lipoteichoic acids flippase LtaA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	289
Detector technology	PIXEL
Collection date	2018-06-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9794
Spacegroup name	C 2 2 21
Unit cell lengths	51.390, 162.470, 191.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.996 - 3.301
R-factor	0.2714
Rwork	0.271
R-free	0.28940
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELXDE
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.996	19.996	3.430
High resolution limit [Å]	3.300	13.300	3.320
Rmerge	0.132	0.050	1.499
Rmeas	0.135	0.052	1.537
Number of reflections	10414	350	747
<I/σ(I)>	12.76	56.16	3.41
Completeness [%]	83.9	97.2	34.6
Redundancy	14	21.266	14
CC(1/2)	0.999	0.999	0.944

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	9.5	289	Glycine: MgAcetate:PEG300