6S76
Crystal structure of human Nek7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-03-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.252, 168.061, 83.443 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.740 - 3.380 |
| R-factor | 0.2633 |
| Rwork | 0.262 |
| R-free | 0.28640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6GT1 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.570 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.740 | 3.500 |
| High resolution limit [Å] | 3.380 | 3.380 |
| Rmerge | 0.101 | |
| Number of reflections | 19383 | 6623 |
| <I/σ(I)> | 6.54 | |
| Completeness [%] | 99.5 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.986 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 20 % w/v Polyethylene glycol 3,350, 150 mM di-Sodium DL-malate; pH 7.0 |
