6S6Q

Crystal structure of the LRR ectodomain of the plant membrane receptor kinase GASSHO1/SCHENGEN3 from Arabidopsis thaliana in complex with CASPARIAN STRIP INTEGRITY FACTOR 2.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-08
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.000006
Spacegroup name	P 43 21 2
Unit cell lengths	192.360, 192.360, 149.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.300 - 2.950
R-factor	0.2124
Rwork	0.209
R-free	0.27970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gr8
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.300	3.030
High resolution limit [Å]	2.950	2.950
Rmeas	0.237	4.540
Number of reflections	59526	4336
<I/σ(I)>	19.1
Completeness [%]	100.0	100
Redundancy	41	40
CC(1/2)	1.000	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	17 % [w/v] PEG 6,000, 0.1 M Tris pH 7.5, 0.2 M LiCl