6S5H
Structure of the human RAB38 in complex with GTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-04-17 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.97624 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 35.804, 144.174, 77.523 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.760 - 2.000 |
| R-factor | 0.1656 |
| Rwork | 0.163 |
| R-free | 0.20940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5oec |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.565 |
| Data reduction software | DIALS |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 38.760 | 38.760 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.130 | 0.077 | 0.649 |
| Rmeas | 0.135 | 0.081 | 0.675 |
| Rpim | 0.037 | 0.023 | 0.185 |
| Total number of observations | 183431 | ||
| Number of reflections | 14044 | 191 | 1005 |
| <I/σ(I)> | 12.2 | ||
| Completeness [%] | 100.0 | 99.3 | 100 |
| Redundancy | 13.1 | 11.2 | 13.1 |
| CC(1/2) | 0.998 | 0.999 | 0.951 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 20 % PEG3350, 10 % ethylene glycol, 0.1 M Bis-Tris-propane pH6 .5, 0.2 M sodium bromide |
