6S51
The crystal structure of glycogen phosphorylase in complex with 10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION SUPERNOVA |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2018-05-29 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.077, 128.077, 115.801 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.740 - 2.370 |
| R-factor | 0.15532 |
| Rwork | 0.153 |
| R-free | 0.20502 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.500 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.740 | 2.460 |
| High resolution limit [Å] | 2.370 | 2.370 |
| Number of reflections | 35501 | 3121 |
| <I/σ(I)> | 13.9 | 2.3 |
| Completeness [%] | 90.5 | 77.2 |
| Redundancy | 4.9 | 3.6 |
| CC(1/2) | 0.996 | 0.883 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | 10 mM BES buffer, pH 6.7 |
