6S4M

Crystal structure of the human organic anion transporter MFSD10 (TETRAN)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9687
Spacegroup name	P 1 21 1
Unit cell lengths	55.994, 70.375, 63.440
Unit cell angles	90.00, 115.45, 90.00

Refinement procedure

Resolution	30.000 - 2.400
R-factor	0.2112
Rwork	0.209
R-free	0.25830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wdo
RMSD bond length	0.007
RMSD bond angle	1.458
Data reduction software	XDS (20190315)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.740	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.208	1.657
Rmeas	0.234	1.853
Rpim	0.104	0.822
Number of reflections	17297	1822
<I/σ(I)>	5.7	1
Completeness [%]	98.7	98.2
Redundancy	4.9	5
CC(1/2)	0.990	0.399

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6.5	293	0.1M ADA pH6.5 -- 0.2M lithium citrate -- 30% PEG400