6S4K
The crystal structure of glycogen phosphorylase in complex with 12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION SUPERNOVA |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2018-05-29 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.5419 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 128.165, 128.165, 115.879 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.520 - 2.430 |
| R-factor | 0.15454 |
| Rwork | 0.152 |
| R-free | 0.21106 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.517 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.520 | 2.530 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Number of reflections | 32703 | 3151 |
| <I/σ(I)> | 16.5 | 3.1 |
| Completeness [%] | 90.0 | 78.6 |
| Redundancy | 5.9 | 4.3 |
| CC(1/2) | 0.997 | 0.901 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 6.7 | 289 | 10 mM BES buffer, pH 6.7 |
