6S45

Room temperature structure of the dark state of the LOV2 domain of phototropin-2 from Arabidopsis thaliana determined with a serial crystallography approach

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	293
Detector technology	PIXEL
Collection date	2018-03-01
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.968
Spacegroup name	P 43 21 2
Unit cell lengths	40.825, 40.825, 133.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.553 - 2.200
R-factor	0.2039
Rwork	0.202
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6qqj
RMSD bond length	0.002
RMSD bond angle	0.547
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.550	2.270
High resolution limit [Å]	2.200	2.200
Rpim	0.051	0.557
Number of reflections	6273	622
<I/σ(I)>	14	1.9
Completeness [%]	100.0	100
Redundancy	87.3	93.9
CC(1/2)	0.999	0.921

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	12-17 % PEG8000 0.2 M Calcium acetate 0.1 M MES