6S3P

Crystal structure of helicase Pif1 from Thermus oshimai in complex with (dT)18

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	60.850, 64.583, 144.296
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.730 - 1.926
R-factor	0.1757
Rwork	0.174
R-free	0.20720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ftd
RMSD bond length	0.009
RMSD bond angle	0.926
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.14rc1_3177: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.730	1.995
High resolution limit [Å]	1.926	1.926
Rmerge	0.056	0.463
Rmeas	0.060	0.509
Rpim	0.023	0.208
Number of reflections	38861	2388
<I/σ(I)>	21.62	4.01
Completeness [%]	88.1	55.48
Redundancy	6.9	5.4
CC(1/2)	0.999	0.937

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	Tris-HCl 0.1M NaF 0.03M NaI 0.03M NaBr 0.03M MES 0.03M PEG 4K 10% Glycerol 20%