6S3E
Crystal structure of helicase Pif1 from Thermus oshimai in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.268, 59.408, 117.150 |
| Unit cell angles | 90.00, 91.82, 90.00 |
Refinement procedure
| Resolution | 36.710 - 3.787 |
| R-factor | 0.2396 |
| Rwork | 0.237 |
| R-free | 0.29470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ftd |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.044 |
| Data reduction software | XDS (Nov 1, 2016, built on 20170215) |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14rc1_3177: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.710 | 3.920 |
| High resolution limit [Å] | 3.780 | 3.780 |
| Rmerge | 0.141 | 1.121 |
| Rmeas | 0.153 | 1.210 |
| Rpim | 0.058 | 0.453 |
| Number of reflections | 10098 | 738 |
| <I/σ(I)> | 12.2 | 2.45 |
| Completeness [%] | 96.7 | 70.4 |
| Redundancy | 7.2 | 6.9 |
| CC(1/2) | 0.996 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | Sodium Hepes-MOPS 0.1M Ethylene glycol 1.25% PEG 4000 20% Glycerol 20% |
