6S34

Zinc free, dimeric human insulin determined to 1.35 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-11
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0000
Spacegroup name	I 21 3
Unit cell lengths	78.170, 78.170, 78.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.080 - 1.350
Rwork	0.161
R-free	0.17740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1b2d
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.080	1.398
High resolution limit [Å]	1.350	1.350
Rmerge	0.058	1.610
Rmeas	0.060	1.656
Rpim	0.014	0.389
Number of reflections	17613	1749
<I/σ(I)>	28.21	1.75
Completeness [%]	100.0	99.94
Redundancy	19.5	18
CC(1/2)	1.000	0.727

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.2 M Sodium Citrate, 0.1 M Sodium Hepes, pH 7.5, 20 % isopropanol