6S2X

Crystal structure of the Legionella pneumophila ChiA C-terminal domain

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-07
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97949
Spacegroup name	C 1 2 1
Unit cell lengths	97.370, 56.640, 128.960
Unit cell angles	90.00, 93.79, 90.00

Refinement procedure

Resolution	64.339 - 1.710
Rwork	0.146
R-free	0.16800
Structure solution method	SIRAS
RMSD bond length	0.008
RMSD bond angle	1.472
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SHARP
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	64.340	1.940	1.750
High resolution limit [Å]	1.710	1.890	1.710
Rmeas	0.066	3.990	0.712
Rpim	0.025	1.159	0.269
Number of reflections	75905	2309	5555
<I/σ(I)>	19.2	1.4	2.8
Completeness [%]	99.9	55	99.9
Redundancy	6.8	11.4	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium acetate, 0.1 M Bis-Tris pH 5.5, 45% (v/v) 2-Methyl-2,4-pentanediol
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M ammonium acetate, 0.1 M Bis-Tris pH 5.5, 45% (v/v) 2-Methyl-2,4-pentanediol