6S2A
PI PLC mutant H82A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.918400 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 130.759, 130.759, 216.898 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.907 - 2.700 |
R-factor | 0.2177 |
Rwork | 0.216 |
R-free | 0.24480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ptd |
RMSD bond length | 0.005 |
RMSD bond angle | 0.827 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.910 | 2.797 |
High resolution limit [Å] | 2.700 | 2.700 |
Number of reflections | 30812 | 2998 |
<I/σ(I)> | 10.95 | |
Completeness [%] | 99.8 | |
Redundancy | 16.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 290 | Morpheus screen (Molecular dimensions) |