6S29

Structure of fission yeast Mis16-Mis19 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-04
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.00004
Spacegroup name	P 1 21 1
Unit cell lengths	53.171, 99.035, 109.880
Unit cell angles	90.00, 98.87, 90.00

Refinement procedure

Resolution	54.280 - 1.988
R-factor	0.1979
Rwork	0.196
R-free	0.23420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6s1l
RMSD bond length	0.009
RMSD bond angle	1.106
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.280	2.060
High resolution limit [Å]	1.988	1.988
Number of reflections	69320	3761
<I/σ(I)>	8.11
Completeness [%]	89.6
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.6 M NaBr, 0.1 M Tris pH 9.0, 20% PEG 3350