6S22
Crystal structure of the TgGalNAc-T3 in complex with UDP, manganese and FGF23c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-14 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.957, 104.823, 143.466 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.010 - 1.960 |
| R-factor | 0.18046 |
| Rwork | 0.179 |
| R-free | 0.21589 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nqa |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.729 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.010 | 2.070 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.414 | |
| Rpim | 0.053 | |
| Number of reflections | 56142 | 8407 |
| <I/σ(I)> | 8.9 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 7.1 |
| CC(1/2) | 0.996 | 0.637 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | Morpheus precipitant mix 4, nitrate phosphate buffer and Morpheus buffer System 1 |
