6S1R

Structure of fission yeast Mis16 bound to histone H4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.8726
Spacegroup name	P 21 21 21
Unit cell lengths	57.390, 77.980, 97.510
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.220 - 1.800
R-factor	0.2059
Rwork	0.204
R-free	0.24070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6s1l
RMSD bond length	0.007
RMSD bond angle	0.909
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15_3459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.220	1.864
High resolution limit [Å]	1.800	1.800
Number of reflections	39885	3857
<I/σ(I)>	9.36
Completeness [%]	96.6
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.05 M LiSO4, 0.8 M NaH2PO4, 1.2 M K2HPO4, 0.1 M CAPS pH 9.5