6S0Y
Nanobody targeting influenza A matrix protein 2 ectodomain (M2e)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-16 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97965 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 57.470, 94.760, 53.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.380 - 1.810 |
| R-factor | 0.18872 |
| Rwork | 0.187 |
| R-free | 0.22529 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hgg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.676 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.060 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rpim | 0.044 | 0.666 |
| Number of reflections | 27557 | 1359 |
| <I/σ(I)> | 11 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13 | 13.2 |
| CC(1/2) | 0.998 | 0.571 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% ethanol, 10% PEG6000, 100 mM Na acetate |
