6S0Y

Nanobody targeting influenza A matrix protein 2 ectodomain (M2e)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-16
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97965
Spacegroup name	P 21 21 2
Unit cell lengths	57.470, 94.760, 53.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.380 - 1.810
R-factor	0.18872
Rwork	0.187
R-free	0.22529
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hgg
RMSD bond length	0.010
RMSD bond angle	1.676
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.060	1.830
High resolution limit [Å]	1.800	1.800
Rpim	0.044	0.666
Number of reflections	27557	1359
<I/σ(I)>	11	1.1
Completeness [%]	100.0	100
Redundancy	13	13.2
CC(1/2)	0.998	0.571

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% ethanol, 10% PEG6000, 100 mM Na acetate