6S0B

Crystal Structure of Properdin in complex with the CTC domain of C3/C3b

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9789
Spacegroup name	P 21 21 21
Unit cell lengths	71.471, 71.497, 134.182
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.960 - 2.312
R-factor	0.2323
Rwork	0.230
R-free	0.27740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.479
Data reduction software	DIALS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.081	2.601
High resolution limit [Å]	2.312	2.312
Rmerge	0.100	1.267
Rmeas	0.111	1.426
Rpim	0.047	0.637
Number of reflections	21145	1538
<I/σ(I)>	11.1	1.5
Completeness [%]	68.6	17.1
Redundancy	5.4	4.6
CC(1/2)	0.996	0.560

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	291	Tacsimate, PEG 3350