6RYP
Bacterial membrane enzyme structure by the in meso method at 2.3 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 54.170, 54.170, 317.540 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.400 - 2.300 |
| R-factor | 0.2557 |
| Rwork | 0.255 |
| R-free | 0.28110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | model from D_1292102692 |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.372 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.400 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmeas | 0.140 | 2.790 |
| Number of reflections | 23038 | 1740 |
| <I/σ(I)> | 7.69 | 0.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.69 | 8.9 |
| CC(1/2) | 0.990 | 0.220 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 100 mM MES/NaOH pH 6.5, 40 %(v/v) PEG400, 400 mM ammonium fluoride and 80 mM magnesium sulfate |
