6RYO

Bacterial membrane enzyme structure by the in meso method at 1.9 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.92003
Spacegroup name	P 32 2 1
Unit cell lengths	52.258, 52.258, 135.882
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.257 - 1.924
R-factor	0.2517
Rwork	0.250
R-free	0.27610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5dir
RMSD bond length	0.002
RMSD bond angle	0.498
Data reduction software	autoPROC
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.257	2.230
High resolution limit [Å]	1.920	1.920
Rmeas	0.170	1.380
Number of reflections	7046	355
<I/σ(I)>	7	1.8
Completeness [%]	86.2	62.5
Redundancy	4.8	3.7
CC(1/2)	0.990	0.470

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	47.1 %(w/v) Polyethylene glycol 1,000, 150 mM Tris/HCl pH 8.0, 80 mM Potassium bromide