6RYN
Structure of conglutinin carbohydrate recognition domain with GlcNAc-alpha-1-phosphate bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.827 |
| Spacegroup name | P 43 |
| Unit cell lengths | 50.278, 50.278, 52.192 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.000 |
| R-factor | 0.1247 |
| Rwork | 0.124 |
| R-free | 0.14000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 6RYJ |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.783 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.1.4) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.304 | 52.276 | 1.000 |
| High resolution limit [Å] | 1.000 | 2.660 | 1.000 |
| Rmerge | 0.040 | 0.030 | 0.389 |
| Rmeas | 0.047 | 0.034 | 0.463 |
| Total number of observations | 538352 | 40167 | 7243 |
| Number of reflections | 70085 | 3636 | 985 |
| <I/σ(I)> | 20.7 | 52.2 | 3.6 |
| Completeness [%] | 99.7 | 94.9 | 99.9 |
| Redundancy | 7.7 | 11 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 2.5 M ammonium sulfate, 0.1 M tris |
