6RYM
Structure of carbohydrate recognition domain with GlcNAc bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-05 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.488 |
| Spacegroup name | P 43 |
| Unit cell lengths | 50.225, 50.225, 51.925 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.106 - 1.460 |
| R-factor | 0.1625 |
| Rwork | 0.161 |
| R-free | 0.18410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 6RYJ |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.380 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.1.4) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.106 | 25.106 | 1.540 |
| High resolution limit [Å] | 1.457 | 4.610 | 1.460 |
| Rmerge | 0.036 | 0.035 | 0.121 |
| Rmeas | 0.054 | 0.063 | 0.186 |
| Total number of observations | 118264 | 4663 | 1857 |
| Number of reflections | 18321 | 739 | 714 |
| <I/σ(I)> | 31.5 | 36.3 | 8.4 |
| Completeness [%] | 80.8 | 97.8 | 21.8 |
| Redundancy | 6.5 | 6.3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 2.5 M ammonium sulfate, 0.1 M tris |
