6RYL

WUS-HD bound to TAAT DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97242
Spacegroup name	P 1 21 1
Unit cell lengths	82.663, 45.914, 87.535
Unit cell angles	90.00, 102.88, 90.00

Refinement procedure

Resolution	41.752 - 2.630
R-factor	0.2385
Rwork	0.237
R-free	0.26360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ry3
RMSD bond length	0.004
RMSD bond angle	0.736
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.920	2.724
High resolution limit [Å]	2.630	2.630
Rpim	0.038
Number of reflections	19403	1907
<I/σ(I)>	11.6
Completeness [%]	99.4
Redundancy	4.5
CC(1/2)		0.552

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1 M CHES (pH 9.5), 20% PEG 8000