6RYI

WUS-HD bound to G-Box DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-08
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.96769
Spacegroup name	C 1 2 1
Unit cell lengths	123.434, 83.068, 85.651
Unit cell angles	90.00, 112.95, 90.00

Refinement procedure

Resolution	40.667 - 2.691
R-factor	0.1971
Rwork	0.194
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ry3
RMSD bond length	0.010
RMSD bond angle	1.105
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.670	2.788
High resolution limit [Å]	2.690	2.691
Rpim	0.052
Number of reflections	21967	2053
<I/σ(I)>	10.97
Completeness [%]	98.1
Redundancy	4.8
CC(1/2)		0.458

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M lithium acetate, 20% PEG 3350