6RYB

Structure of deubiquitinase for PR-ubiquitination 1 -Dup1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	87.259, 87.259, 612.618
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.589 - 2.315
R-factor	0.2184
Rwork	0.217
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6b7p
RMSD bond length	0.008
RMSD bond angle	0.938
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX ((1.12_2829: ???))
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.590	2.398
High resolution limit [Å]	2.315	2.315
Rmerge	0.125	2.296
Rmeas	0.128	2.348
Rpim	0.027	0.488
Number of reflections	62273	5993
<I/σ(I)>	19.11
Completeness [%]	99.9
Redundancy	21.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	18 - 20 % PEG3350 / PEG4000, 100 mM HEPES pH 7.5, 200 mM Ammonium sulfate