6RY7
Crystal structure of Dfg5 from Chaetomium thermophilum in complex with laminaribiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-06 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.972 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.356, 54.879, 80.095 |
| Unit cell angles | 90.00, 90.45, 90.00 |
Refinement procedure
| Resolution | 40.046 - 1.300 |
| R-factor | 0.095 |
| Rwork | 0.094 |
| R-free | 0.11920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.050 | 1.346 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.026 | 0.099 |
| Rmeas | 0.035 | |
| Number of reflections | 86591 | 8648 |
| <I/σ(I)> | 16.58 | 6.88 |
| Completeness [%] | 97.4 | 97.52 |
| Redundancy | 2 | 1.9 |
| CC(1/2) | 0.998 | 0.974 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291.15 | Ammoniumiodide, PEG3350, Calcium acetate, MES, PEG 8000 |
