6RXI
In-flow serial synchrotron crystallography using a 3D-printed microfluidic device (3D-MiXD): Lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2018-02-13 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.700, 79.700, 38.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 56.360 - 2.000 |
| R-factor | 0.15984 |
| Rwork | 0.158 |
| R-free | 0.20117 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6h79 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.584 |
| Data reduction software | CrystFEL (0.8.0) |
| Data scaling software | CrystFEL (0.8.0) |
| Phasing software | MOLREP (11.7.01) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.360 | 2.080 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 8876 | 869 |
| <I/σ(I)> | 6.21 | 1.83 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 625 | 448 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 3 | 293 | 30 mg/mL lysozyme in 100 mM NaAc (pH3.0) rapidly mixed in a 1:1 ratio with precipitant (100 mM NaAc pH 3.0, 20% NaCl, 6% PEG 6000) |
