6RVQ
SaFtsz-GDP-EthGLy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.373, 50.500, 88.431 |
| Unit cell angles | 90.00, 111.52, 90.00 |
Refinement procedure
| Resolution | 33.464 - 1.136 |
| R-factor | 0.2078 |
| Rwork | 0.207 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rvn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.113 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.200 |
| High resolution limit [Å] | 1.136 | 1.140 |
| Number of reflections | 108093 | 15595 |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 99.1 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.998 | 0.716 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 27.5 % PEG 5K MME, 10 % EthGly, 0.2 M Li2SO4, 0.1 M Tris pH 9.0 |
