6RVP
SaFtsz-GDP-MetPyr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9790 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.477, 50.079, 88.502 |
| Unit cell angles | 90.00, 111.78, 90.00 |
Refinement procedure
| Resolution | 36.207 - 1.160 |
| R-factor | 0.1916 |
| Rwork | 0.191 |
| R-free | 0.20830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6rvn |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.115 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.220 |
| High resolution limit [Å] | 1.160 | 1.160 |
| Number of reflections | 92535 | 8143 |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 91.0 | |
| Redundancy | 13.4 | |
| CC(1/2) | 0.999 | 0.635 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 26% PEG 5K MME, 10% EthGly, 0.2 M LI2SO4, 0.1 M Tris pH 8.7 |
