6RVC
Crystal structure of Patched-1 ectodomain 2 (PTCH1-ECD2) in complex with nanobody 75
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.180, 101.120, 106.180 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.160 - 2.200 |
| R-factor | 0.20562 |
| Rwork | 0.204 |
| R-free | 0.24073 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6dmy |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.455 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.160 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.164 | |
| Rpim | 0.059 | 0.722 |
| Number of reflections | 42185 | 3069 |
| <I/σ(I)> | 9.8 | 1.1 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 8.6 | 8.3 |
| CC(1/2) | 0.997 | 0.391 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M sodium nitrate, 20% (w/v) PEG 3350 |
