6RV6

Structure of properdin lacking TSR3 based on anomalous data

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	2.47968
Spacegroup name	I 41
Unit cell lengths	219.790, 219.790, 47.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.707 - 3.507
R-factor	0.2649
Rwork	0.264
R-free	0.28350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	electron density for TB-TSR1-TSR5-TSR6
RMSD bond length	0.007
RMSD bond angle	1.384
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	155.000	3.600
High resolution limit [Å]	3.507	3.507
Number of reflections	27811	2051
<I/σ(I)>	12.2
Completeness [%]	99.9
Redundancy	20.07

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4	292	1.0 M lithium sulfate, 0.1 M sodium acetate pH 4.0, 0.1 M barium chloride