6RV5

X-ray structure of the levansucrase from Erwinia tasmaniensis in complex with levanbiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-13
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	127.886, 127.886, 58.268
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.260 - 1.580
R-factor	0.12623
Rwork	0.146
R-free	0.19290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6frw
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.420	1.640
High resolution limit [Å]	1.450	1.580
Rmerge	0.027	0.287
Rmeas	0.038	0.406
Rpim	0.027	0.287
Number of reflections	66162	6517
<I/σ(I)>	2.04
Completeness [%]	99.5
Redundancy	1.3
CC(1/2)	0.999	0.888

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293.15	28% Glycerol, 14% PEG 4000, 1.5mM Manganese(II) chloride tetrahydrate, 1.5 mM Cobalt(II) chloride hexahydrate , 1.5 mM Nickel(II) chloride hexahydrate, 1.5 mM Zinc acetate dihydrate crystals soaked in Sucrose 500 mM